Vaccine against HIV / AIDS "DNA-4"

Vaccine against HIV / AIDS
in the pipeline

Description

Genome HIV-1 (consensus sequence HIV-1 subtype A-EE

Vaccine DNA-4 contains equimolar amounts of four plasmid DNA in saline NaCl with the total concentration of 1 mg / ml

Scheme of purifying DNA vaccine

Parameter

Method

Criteria for DNA vaccines

supercoiled DNA

Agarose gel electrophoresis

>90%

RNA admixture

Agarose gel electrophoresis

-

DNA E.coli admixture

Southern blotting

<10 ng / ml

(5,7±3,6 ng / ml)

Purity

spectrophotometric analysis, λ=210-300 ng

A260/A280>1.8

A260/A230>1.9

 

Protein admixture

BCA test

<10 ng / ml

(6±4 ng / ml)

Endotoxin content

LAL test

<0.1 EU / mg

(0.04±0.03 EU / mg)

Аuthenticity of plasmids

PCR sequencing

 

Preclinical studies

Security Rating:

• "DNA 4" is not toxic for laboratory animals in acute and chronic toxicity tests

• "DNA 4" refers to a class of 5 practically nontoxic substances

• Lethal doses of  LD50, LF16 and LD84 are impossible to be reached

• "DNA 4" is not allergenic

• "DNA 4"does not have pyrogenic effects

Project director